A relatively short-lived species, the Arno goby usually begins breeding at around one year of age (2). Spawning occurs between April and July, with the exact timing varying according to the temperature of the water (1) (3).
The male Arno goby produces a variety of sound signals during courtship of the female (4) (6) (7). This species is extremely territorial during the breeding season (2) (3) (7), and the male also demonstrates considerable acoustic activity during the spawning period and when defending the nest or shelter (2) (6) (8). The female Arno goby deposits eggs between stones, shells and aquatic plants on the river bed, and the eggs are guarded by the male until they hatch after five to six days (2) (3) (4).
The Arno goby feeds on a wide variety of small invertebrates, fish eggs and insect larvae (2) (3) (4).